FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004853948

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004853948
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46198620
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG726380.15722980469979406No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG221400.017.43688245
GTTGTCG37800.016.8434626
GGCATAG236300.016.34726735
ATAGGGG255800.015.51444138
GGGAGGT350450.015.4648013
GGAGGTC338150.015.3016854
GAGCTTA257750.015.14402244
GTCACTC332300.015.0685618
GGTCACT338700.014.8438857
TGGCATA266950.014.64770834
TGTCGTT43900.014.50343328
ACTCTCC348050.014.44897311
CATAGGG278450.014.01798737
ATATAGC51150.013.8598096
AGGTCAC384050.013.6774586
GAGGTCA384600.013.6755565
GCATAGG292200.013.43546936
TAGCGTA9100.013.35013937
CACTCTC393100.012.74739810
GCTAAGT51100.012.63700714
Produced by FastQC (version 0.11.5)