FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004853950

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004853950
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50500364
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCATAG149150.014.40875135
AGCTTAG151500.014.33189445
ATAGGGG157900.013.76531138
GGGAGGT260050.012.7739883
CATAGGG171050.012.58930937
GGAGGTC256450.012.531554
TGGCATA182400.011.893887534
GTCACTC264950.011.8928548
GCATAGG184100.011.69756136
GGTCACT268250.011.6792887
GAGCTTA189800.011.48726244
ACTCTCC278600.011.37581911
TCAGACG80050.011.0412912
CGTAGGG81650.010.72028837
GGCGTAG82200.010.70412535
GCGTAGG81900.010.68815836
CGTCGAT25300.010.58033712
TAGGGGA299950.010.11166339
ACCCGGC75000.010.0756415
ATCCTAG116450.09.8111366
Produced by FastQC (version 0.11.5)