FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004853953

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004853953
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42637968
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGACG23200.016.9699296
GTCCGTT36450.016.4349961
TCGACGA26250.015.8552257
GCACTAC54350.015.65126925
ACGTTCG12900.015.52137856
CCCGTAT27100.015.1247291
CGACGAG25350.015.0869498
GTCCGAT22750.014.5519581
GTCCGCT37300.014.2491421
TACGGTA55050.014.01834744
AACGCTA115200.013.98448823
CCCCCCT41400.013.9803581
TCACGCT122250.013.9308468
CTCCGTT67100.013.8280271
CTCCGGT56250.013.6526631
ACGCTAC118250.013.60552624
GTCGGTT35600.013.6010981
CCTTACG57250.013.5975841
CCCGCGT11850.013.4935271
CCCGGAT90750.013.4753071
Produced by FastQC (version 0.11.5)