FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004853955

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004853955
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48043192
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCAGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG895810.18645930103894845No Hit
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG726370.15119103659890043No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGCTA311200.026.67859323
ACGCTAC312350.026.57316824
TCACGCT329750.025.7441258
CGCTACC323750.025.54711525
AAAACGC330700.025.15309321
GAAAACG329000.024.80434820
AAACGCT351200.023.7297222
TTCACGC374900.022.9317787
ACGCTCA390300.022.6322610
CACGCTC387050.022.0375279
GTTTTCG379000.021.8113945
TCGTGGT389750.021.34840840
CGTGGTT403800.020.72827541
ACCGCGT122850.020.6041156
GACCGCG122650.020.6010885
AGACCGC122800.020.5212974
CTACCTC414700.020.26979827
TTCGACG21700.020.1149336
GCTACCT428450.019.67703626
TACCTCT439000.019.22468228
Produced by FastQC (version 0.11.5)