FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004853956

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004853956
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48043192
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG832370.17325451647758958No Hit
TTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAA553920.11529625258871225No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG264450.016.0441445
GTCACTC390000.015.8107728
GGGAGGT421200.015.38931753
GGTCACT400650.015.3626477
AGGTCAC417950.015.1413586
GGAGGTC415650.015.008144
GAGGTCA418650.015.0077495
GGCATAG284500.014.85825635
ACTCTCC426550.014.37980211
ATAGGGG295600.014.2545238
GTTGTCG25000.014.12819126
GAGCTTA303950.014.03311444
TGGCATA303700.013.91891234
TCACTCT469750.013.2749739
TGGGAGG338700.012.9962752
ACCCGGC97700.012.9724095
CACTCTC480200.012.81151510
CATAGGG343950.012.414227537
CTGGGAG394600.012.234221
TAGCGTA10650.011.8292537
Produced by FastQC (version 0.11.5)