FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004853957

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004853957
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48451141
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT1239650.25585568769164796No Hit
CTCAACTTTCTTGTCCACCTTGGTGTTGCTGGGCTTGTGATTCACGTTGCA644710.13306394580057465No Hit
CCACGGTGAGCTTGCTGTAGAGGAAGAAGGAGCCGTCGGAGTCCAGCACGG501330.10347124745730962No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGCGT218050.027.2823946
AGACCGC219700.027.0369534
TTCGACG22750.026.1095666
GAACGAT223900.026.02727145
CCGCGTT229300.025.9045547
CAGACCG231200.025.8672943
CGCGTTC237350.025.054418
GACCGCG242050.024.5679665
TCAGACC263750.022.9992662
AGAACGA259150.022.66076344
AATACGG262500.022.49147432
ATACGGA264300.022.31278233
TACGGAG265450.022.18223634
GCGTTCT276800.021.5648989
AACGCTA209500.021.03907623
ACGCTAC211950.020.80649424
CAATACG288200.020.57139831
TCACGCT224900.020.2887258
CGCTACC219300.020.04758825
ACGGAGA295800.019.99754735
Produced by FastQC (version 0.11.5)