FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004853958

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004853958
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48451141
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG603410.12453989473643148No Hit
TTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAA505040.10423696729866486No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG165550.018.44050645
GGCATAG176350.017.23466935
GTTGTCG33750.016.66471126
GAGCTTA194550.015.74952944
TGGCATA196400.015.50958734
ATAGGGG200200.015.3049738
ACCCGGC90450.015.1567695
CATAGGG206850.014.72592537
ATATAGC44850.014.4008816
TGTCGTT39650.014.24160528
GCATAGG217900.014.08251436
TCACCCG101800.013.8885623
TATAGCT45600.013.8679137
GCTAAGT41850.013.81616914
GGGAGGT332350.013.5684643
ATAGCTC92500.013.52233239
ACGGACA104500.013.28356918
GGAGGTC318600.013.2555294
AGCGCTC70000.012.8272019
TAGCTCT100750.012.48205840
Produced by FastQC (version 0.11.5)