FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004853960

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004853960
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49892491
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG717730.14385531482082142No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG218550.017.13015745
ATAGGGG234100.016.42165438
GGGAGGT320050.016.1962973
GGCATAG234500.016.01978135
GGAGGTC325950.015.4897044
GAGCTTA260150.014.632668544
GGTCACT341050.014.5192847
TGGCATA262000.014.48417234
GTCACTC340900.014.482028
GTTGTCG36600.014.386806526
ACTCTCC343350.014.30696811
CATAGGG271600.013.9968537
GAGGTCA374300.013.6066885
GCATAGG284850.013.34553536
TGGGAGG312900.012.9344222
AGGTCAC398000.012.7560896
TCACTCT395950.012.5643139
ATATAGC46000.012.5184686
CACTCTC397650.012.27165510
TGTCGTT43550.012.19708428
Produced by FastQC (version 0.11.5)