FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004853961

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004853961
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46389300
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT2286630.49292185913561964No Hit
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG1966510.42391456650563814No Hit
CTTCCGTACGCCACATGTCCCGCGCCCCGCCGCGGGGCGGGGATTCGGCGC1020520.21999038571394697No Hit
CTGGGCTCTTCCCTGTTCACTCGCCGTTACTGAGGGAATCCTGGTTAGTTT923720.19912350477373017No Hit
CCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGATCATCAA853540.18399501609207294No Hit
CCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCGG549110.11836996893680224No Hit
CTCCCCAATACGGAGAGAAGAACGATCATCAATGGCTGACGGCAGTTGCAG507290.1093549590099441No Hit
ACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGA473160.10199765894290279No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGACCG450900.026.0801473
AGACCGC450150.026.0731984
CGGTATA36650.025.90170720
GACCGCG462000.025.4145745
ACCGCGT487450.024.0836
TCAGACC503600.023.7623672
TCGACGA31800.022.2811097
ACGCTAT63950.021.42657524
CTCAGAC607050.021.127671
GTTCGAC34000.020.4424935
ACGCTAC536400.020.23459624
AAAACGC608300.020.15196621
AACGCTA607850.020.12475223
GAAAACG614050.019.9729420
CTACACG122150.019.96368445
GTTTTCG614350.019.78078845
AAACGCT621000.019.77344722
TCACGCT631250.019.683638
GCCGGCT570000.019.6478421
CGTGGTT627450.019.49648341
Produced by FastQC (version 0.11.5)