FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004853962

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004853962
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46389300
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG1349970.2910089180047985No Hit
TTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAA1291200.2783400482438838No Hit
CATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCG1111310.2395617092734747No Hit
CCGAAAACCACGAGGAAGAGAGGTAGCGTTTTCTCCTGAGCGTGAAGCCGG1037430.22363562286992905No Hit
GTTTCTCTGAACGTGTAGAGCACCGAAAACCACGAGGAAGAGAGGTAGCGT1005300.21670945670661124No Hit
CGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAGAAGAAACT671470.14474674116660524No Hit
CTCTGAACGTGTAGAGCACCGAAAACCACGAGGAAGAGAGGTAGCGTTTTC650760.14028234959354852No Hit
CTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAAAAC597750.12885514547535745No Hit
GCTGAATTTAAGCATATTAGTCAGCGGAGGAGAAGAAACTAACCAGGATTC587520.1266498955578118No Hit
CAGGGATCATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAG542530.11695153839355195No Hit
ATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGA537700.11591035001605975No Hit
CGCTGAATTTAAGCATATTAGTCAGCGGAGGAGAAGAAACTAACCAGGATT532070.11469670807707812No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGAGGT526400.017.4569783
GGAGGTC526100.017.1849544
GGTCACT526150.016.9468737
GTCACTC528850.016.7577388
AGCTTAG422250.016.04916645
ACTCTCC557700.015.8262511
TGGGAGG458300.015.6347922
TCAGACG177800.015.5643222
CTGGGAG492700.015.4231331
AGGTCAC587050.015.2966566
TCACTCT592950.015.0711599
ATATAGC39850.014.9364136
CACTCTC593950.014.91332810
GAGGTCA623650.014.5254375
CACCCGG121850.014.3603521
GGCATAG478700.014.32839435
ACGAACG42050.014.15230419
AGGGATC224850.013.8658932
GAGCTTA492650.013.78478944
AGGCTCT506150.013.72278820
Produced by FastQC (version 0.11.5)