FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004853964

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004853964
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences52363266
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCCGGC101600.014.2362865
TCACCCG108200.013.9108583
AGCTTAG152150.013.80558245
GGCATAG161550.012.99695235
GTTGTCG28900.012.93024426
ACGGACA112500.012.46175118
CCGGACA117450.012.29200612
CGGACAC116700.012.20162613
ATAGGGG179350.011.80542238
CACCCGG133200.011.5193984
GAGCTTA186100.011.49362844
TGGCATA185550.011.46143234
CATAGGG188500.011.25742137
ATAGCTC122350.011.19935239
ACACGGA127600.011.19867316
CGGACAG127250.010.99958119
GGGAGGT306350.010.9647053
GCCCGGA139900.010.75065610
GCATAGG197750.010.70808236
GGAGGTC283050.010.6430734
Produced by FastQC (version 0.11.5)