FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004853965

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004853965
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48504126
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT1343050.2768939698037235No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACCGCG243250.027.7483225
ACCGCGT243900.027.711246
GAACGAT247750.027.1353145
AGACCGC250700.027.04044
CCGCGTT254350.026.6169517
CAGACCG259750.026.1069413
CGCGTTC260250.026.0048858
TCAGACC294100.023.6697442
AGAACGA284750.023.66469644
ATACGGA292700.022.8070933
AATACGG296850.022.44276632
TACGGAG296200.022.37047634
GCGTTCT309500.021.9322179
AAGAACG307150.021.8729443
CAATACG314350.021.4152531
ACGGAGA325700.020.44100435
CTCAGAC379600.020.3346181
TTCGACG18200.019.9031756
CCCAATA357600.019.08939729
CGGAGAG352100.018.7547536
Produced by FastQC (version 0.11.5)