FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004853968

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004853968
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50832041
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG131200.013.70690445
GGCATAG139250.012.84669835
ATAGGGG151900.012.02964738
CATAGGG152600.012.00341537
GAGCTTA156750.011.53020444
GCATAGG161600.011.26496236
GTTGTCG32550.011.1273126
ACCCGGC97450.010.6895375
GTCACTC231200.010.6261258
ATAGCTC91150.010.56757839
GGTCACT226000.010.5621787
CACCGAA185150.010.44229345
TGGCATA177250.010.28295534
GCACCGA182950.010.24805844
GGGAGGT280050.010.1952083
AGGTCAC246750.010.1298696
ACTCTCC246700.010.04082411
GGAGGTC264300.09.883374
ACGGACA102200.09.73034318
TCACCCG111650.09.45113853
Produced by FastQC (version 0.11.5)