FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004853970

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004853970
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50218760
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG574960.11449107863276593No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG176200.015.67313245
GGCATAG199000.013.95026535
ATAGGGG210950.013.3523938
GAGCTTA214800.013.06437944
GTCACTC276300.013.0406718
CATAGGG222850.012.55829537
GGTCACT287150.012.5319527
AGGTCAC294200.012.4065716
GGGAGGT320500.012.3276293
TGGCATA228900.012.30492334
GCATAGG233450.011.98800436
ACTCTCC309300.011.88944511
GGAGGTC312600.011.8334524
GAGGTCA317950.011.7908975
GTTGTCG28500.010.73564826
TCACTCT345650.010.567489
ACCCGGC85850.010.248665
CACTCTC355100.010.17217410
TAGGGGA355800.010.0981839
TCTCCCC394350.09.519052513
Produced by FastQC (version 0.11.5)