FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004853971

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004853971
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences60443214
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGACG37100.024.734846
CGGTATA67250.021.57468820
ACCGCGT61750.020.3609336
TCGACGA48150.019.3853477
AACGCTA170350.018.84594223
ACGCTAC170150.018.82889624
GACCGCG67400.018.7431875
AGACCGC68550.018.52734
CGACGAG50000.018.1282888
CGCTACC177900.018.00874125
CCGCGTT73900.016.9524527
TCACGCT206500.016.827918
AAAACGC195200.016.64079921
CGCGTTC76950.016.3097528
TCGGCGC109300.016.07489845
GTCCGTT44850.015.82512951
TTCGGCG113450.015.64588944
CAGACCG83750.015.5698683
CCCGTAT34300.015.4373551
ATGACGG90900.015.31674616
Produced by FastQC (version 0.11.5)