FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004853972

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004853972
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences60443214
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCATAG177100.013.43625435
AGCTTAG179400.013.13231145
ATAGGGG188200.012.81149838
TCAGACG114500.012.0253782
GTCACTC268650.011.9778
GGTCACT264200.011.9487847
GGGAGGT297700.011.7730113
CATAGGG205800.011.63928537
AGGTCAC286900.011.434716
GAGCTTA210950.011.32750244
GGAGGTC295350.011.1678514
GAGGTCA303750.011.0812925
ACCCGGC106200.011.080325
TGGCATA218100.011.05088434
GCATAGG216450.010.98338936
ACTCTCC300250.010.83614411
TAGCGTA16250.010.11345537
ACGGACA117700.09.9551918
TCACCCG128950.09.6297873
AACTCTA134650.09.54367845
Produced by FastQC (version 0.11.5)