FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004853975

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004853975
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50014179
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT957800.19150569281563135No Hit
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG651570.13027705603245032No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGACG55950.032.1663486
TCGACGA62550.028.9161597
CGACGAG63850.027.8693898
GTTCGAC67350.027.055735
CGGTATA79600.026.76391420
AGACCGC217550.024.4662554
ACCGCGT217000.024.3001926
GACCGCG215850.024.283755
CAGACCG232150.023.0341573
CCGCGTT232450.022.7140927
CGCGTTC240200.022.0374228
AGTTCGA90950.020.8267364
TCAGACC277000.020.279192
ATGACGG102750.019.90193216
GAACGAT266050.019.49369645
GCGTTCT275600.019.3455479
CGAGGAT102800.019.32321711
GACGGTA110050.019.19502618
GTATAGA123400.019.01543222
CTCAGAC335600.019.0015491
Produced by FastQC (version 0.11.5)