FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004853978

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004853978
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences52220088
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG592490.11346016881472892No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG186750.016.85243645
ATAGGGG193350.016.38830638
GGCATAG194450.016.30834635
GGGAGGT306850.014.7499433
GAGCTTA217700.014.5901944
TGGCATA221850.014.42646734
GGAGGTC314400.014.1385564
CATAGGG226250.014.07581337
GTCACTC326250.013.3345188
GCATAGG241350.013.23245136
GGTCACT332350.013.101647
ACTCTCC334900.013.02109711
GTTGTCG23300.011.97555226
TGGGAGG296250.011.6912892
TAGGGGA366350.011.63452239
CACTCTC381000.011.48913510
AGGTCAC396700.011.296736
GAGGTCA404300.011.26713855
TCACTCT407450.010.9640899
TCTCCCC403300.010.80973213
Produced by FastQC (version 0.11.5)