FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004853979

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004853979
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47663873
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGGCTCTTCCCTGTTCACTCGCCGTTACTGAGGGAATCCTGGTTAGTTT839180.1760620669663164No Hit
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG715520.15011788907712137No Hit
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT579920.1216686692665533No Hit
GGCGGACTTTGGAGTGGGAAGCGGGGAATGTCTACGTGCGTATGCACGTGG529920.1111785439676713No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGCGT142950.021.1176726
AGACCGC143800.021.0556164
GACCGCG147650.020.445545
TTCGACG19700.020.0966726
CAGACCG159050.019.362063
TCACGCT327500.019.2938168
CCGCGTT158100.019.0656057
CCGGCTT359300.018.6068732
GCCGGCT323500.018.5086961
GTTAGTT238650.018.49636744
TTCACGC346650.018.429137
CGCGTTC166700.018.0955128
ACGCTAC344350.018.0437124
AACGCTA345050.018.02611723
GCGGACT159100.017.814852
GAAAACG328800.017.24043720
CACGCTC373450.017.0946759
CGGCTTC400300.017.0438863
ACGCTCA380150.017.03599710
CGCTACC271000.017.00885225
Produced by FastQC (version 0.11.5)