FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004853982

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004853982
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48450254
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAA574890.1186557246944464No Hit
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG571000.11785283932670405No Hit
CATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCG528700.10912223494225644No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG197750.014.78271545
ACCCGGC85150.013.6297285
GGGAGGT299300.013.5539613
GGCATAG214400.013.54403435
TCACCCG90050.013.067583
GGAGGTC293600.013.0641394
ATAGGGG229800.012.92440538
ACTCTCC293450.012.67959711
GAGCTTA238450.012.39342744
TGGCATA237300.012.36989734
GTTGTCG22100.012.22319226
GTCACTC303300.012.1600898
TCAGACG116050.011.9618752
CATAGGG247150.011.8088837
GGTCACT313600.011.80534657
CGGACAC100600.011.38140613
GCATAGG255300.011.38023136
CCGGACA104850.011.2594912
AGGGATC146100.011.2262962
ACGGACA100000.011.20575518
Produced by FastQC (version 0.11.5)