FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004853983

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004853983
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47299061
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT2357500.4984242710441968No Hit
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG1569060.33173174410375716No Hit
ACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGA537640.11366821848746637No Hit
CACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACG531140.1122939840179914No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAACGAT504600.027.23163645
AGACCGC566050.024.4149784
ACCGCGT563850.024.327226
GACCGCG564400.024.3036425
CAGACCG570350.024.3022563
CCGCGTT576050.023.8199357
AGAACGA587900.023.46814744
TCAGACC612700.023.0704232
AACGCTA385400.022.87443523
ACGCTAC388350.022.6850224
CGCGTTC607150.022.5664428
CGCTACC394600.022.24891725
GCCGGCT367800.022.0618571
AAAACGC400850.022.01514621
GCGTTCT632600.021.708919
CTCAGAC672950.021.6988371
TCACGCT411400.021.6904418
GAAAACG408600.021.48624820
TTCACGC424050.021.2821147
AAACGCT417500.021.16230822
Produced by FastQC (version 0.11.5)