FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004853984

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004853984
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47299061
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAA970780.2052429751195272No Hit
CATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCG920170.19454297411950736No Hit
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG705570.14917209455807168No Hit
CCGAAAACCACGAGGAAGAGAGGTAGCGTTTTCTCCTGAGCGTGAAGCCGG522660.11050113658704556No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG217900.016.8448745
ATAGGGG235450.015.67187238
GGCATAG237050.015.47151935
GAGCTTA251900.014.58083744
GTCACTC352650.014.4590178
GGGAGGT383750.014.2510853
TGGCATA259850.014.18234534
AGGTCAC372850.013.9835076
GGAGGTC390950.013.7010544
GAGGTCA389200.013.6990525
ACTCTCC378800.013.34806811
GGTCACT387100.013.3290947
GTTGTCG40650.013.06168726
CATAGGG285700.012.98690837
TCACTCT406750.012.5688889
TGTCGTT44100.012.22890428
AGGGATC209000.012.218362
GCATAGG304350.012.20607236
CACTCTC410800.012.15526410
TGGGAGG340000.011.7185372
Produced by FastQC (version 0.11.5)