FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004853986

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004853986
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46776770
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAA758350.16212106992423805No Hit
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG716380.1531486675971855No Hit
CATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCG660880.14128380390522902No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG223900.016.98866345
GGCATAG243650.015.57293235
ATAGGGG254850.015.09716438
GGGAGGT351050.014.7712583
GTCACTC337750.014.6948638
GAGCTTA260800.014.67143344
GGTCACT347400.014.2932737
TGGCATA274750.013.92661334
ACTCTCC353250.013.87836911
GGAGGTC367700.013.8218484
AGGTCAC369600.013.7201776
CATAGGG282150.013.50857237
GAGGTCA394750.012.9996975
GCATAGG298800.012.83189736
TCACTCT393800.012.6090279
CACTCTC393900.012.3721610
TGGGAGG332750.012.3184022
TGCCCGT184050.012.14764644
AGGGATC169550.011.9685732
TCAGACG97650.011.6778562
Produced by FastQC (version 0.11.5)