FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004853987

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004853987
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences53402596
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGTAT36950.018.059771
GTCCGTT48650.016.8212681
ACGTTCG22450.015.9338466
GTTAGTT135600.015.01514644
ACGAACG51150.014.91236131
TCACGCT150550.014.7345228
TACGGTA79500.014.71556344
ACGGTAC79950.014.68901545
AACGCTA117150.014.51980723
ACGCTAC118050.014.40921824
CGTGCGA24400.014.199717538
TTACGGT84050.014.07954843
GTCCGAT31350.013.9508011
GTCGGTT41900.013.8816371
CGCTACC123500.013.68239225
GCGGACT84600.013.5918012
GCACTAC70850.013.52878625
GCCGGCT185050.013.4741391
CCTTACG87200.013.46773941
TACGTGC75950.013.45005533
Produced by FastQC (version 0.11.5)