FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004853988

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004853988
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences53402596
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG639370.11972638933133513No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG187300.016.97070345
GGCATAG215550.014.81366635
GAGCTTA222400.014.31268744
ATAGGGG224850.014.24044238
GGTCACT325750.013.3822327
GTCACTC330050.013.2878128
CATAGGG240900.013.26415937
GGGAGGT357800.013.2185663
TGGCATA247800.012.985723534
AGGTCAC346550.012.8645966
GAGGTCA352850.012.8466825
ACTCTCC345000.012.7176211
GCATAGG257800.012.49064536
GGAGGTC365000.012.3652334
TAGGGGA388100.011.11941339
ACCCGGC94300.010.7589745
GCGTTCG22950.010.68606515
TGGGAGG317850.010.6184562
AGGGGAG418200.010.49654740
TCAGACG101200.010.47207452
Produced by FastQC (version 0.11.5)