FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004853989

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004853989
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45916682
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG1497930.3262278402433347No Hit
GGGCTCTTCCCTGTTCACTCGCCGTTACTGAGGGAATCCTGGTTAGTTTCT691480.15059450506462987No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT495920.10800431965010016No Hit
GGGGATTCGGCGCTGGGCTCTTCCCTGTTCACTCGCCGTTACTGAGGGAAT466880.10167982085465146No Hit
GCTGGGCTCTTCCCTGTTCACTCGCCGTTACTGAGGGAATCCTGGTTAGTT459450.10006167257468647No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGCTA275600.026.8487423
GCCGGCT337950.026.6916471
ACGCTAC279400.026.48470724
CGCTACC280400.026.3180725
AAAACGC291950.025.35892321
CCGGCTT329400.025.2463172
TCACGCT303250.024.6281938
GAAAACG302000.024.26164820
AAACGCT317850.023.59840422
GCCGTAT50600.023.5794581
CCTTACG91300.023.35891541
TTCACGC321000.023.1892667
CACGCTC328600.022.5981679
ACGCTCA331450.022.45136610
CGTGGTT333050.022.16215141
GTTTTCG328700.022.15224845
CGGCTTC377150.022.1275233
TCGCGAT27150.022.04085238
TTCGCGA27600.022.00738137
TACGGTA98000.021.96833844
Produced by FastQC (version 0.11.5)