FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004853992

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004853992
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47607222
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG520240.10927753776517352No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCCGGC118600.019.0289445
TCACCCG125500.018.5177923
ACGGACA130500.017.25598118
CCGGACA132950.016.82352812
AGCTTAG160500.016.40933645
CACCCGG141550.016.3554614
CGGACAG139350.016.04665819
GGCATAG164250.016.00469635
CGGACAC139700.015.96232913
GCCCGGA156550.014.73250410
TGGCATA179850.014.67898334
ATAGCTC148300.014.64747839
ACACGGA152400.014.60048416
GAGCTTA183200.014.560081544
CACGGAC155000.014.50069317
TAGCGTA19950.014.20217837
CATAGGG189750.013.91277137
GTTGTCG23300.013.82931226
TAGCTCT157800.013.79469640
TGATAGC164300.013.53586737
Produced by FastQC (version 0.11.5)