FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004853995

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004853995
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences52110410
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT992890.19053582575919092No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAACGAT191100.025.02235845
ACCGCGT216600.022.1881526
AGACCGC219000.022.0680474
GACCGCG221000.021.8175585
CCGCGTT222400.021.6297387
CGCGTTC228200.021.1292888
AGAACGA231950.020.67350444
CAGACCG240050.020.1797433
AATACGG250200.019.12725632
ATACGGA252250.019.01640933
TACGGAG253450.018.91749634
AAGAACG260300.018.43023743
TCAGACC274150.017.9077012
CAATACG270050.017.76296631
GCGTTCT281800.017.2061869
AACGCTA86100.017.1424623
ACGCTAC87050.017.08461424
ACGGAGA286950.016.92067535
CGGAGAG303350.015.90205436
CGCTACC94550.015.72940925
Produced by FastQC (version 0.11.5)