FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004853996

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004853996
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences52110410
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG133200.013.78693945
GGCATAG143250.012.95110935
ATAGGGG146700.012.873610538
CATAGGG154100.012.18082737
GCATAGG163300.011.50629636
GAGCTTA163950.011.46556844
GTCACTC216600.011.4571968
GGTCACT216050.011.3827967
TGGCATA179450.010.551270534
AGGTCAC245400.010.4419476
GGGAGGT262250.010.4411673
ACTCTCC246900.010.31602411
GGAGGTC255500.010.2510534
GAGGTCA269850.09.9630755
ACCCGGC77950.09.7391765
TAGGGGA262650.09.17498139
CGTCGAT23000.08.78934112
TCAGACG84450.08.6975892
TGCCCGT135100.08.58558244
TCACTCT299800.08.51750859
Produced by FastQC (version 0.11.5)