FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004853997

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004853997
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47752055
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT780340.16341495669662803No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACCGC151400.026.1328644
GACCGCG158300.024.9653475
ACCGCGT160450.024.5903476
CAGACCG167600.023.7953173
CCGCGTT169650.023.2568287
GAACGAT168100.022.90493645
CGCGTTC176000.022.417718
AATACGG182050.021.11949232
TACGGAG183900.020.8584534
ATACGGA184500.020.8393933
AGAACGA190550.020.37684644
TCAGACC200300.020.1915992
AACGCTA125950.020.16373623
ACGCTAC128350.019.82170724
CAATACG203800.019.05323431
CGCTACC135300.018.73701925
AAGAACG212450.018.20234543
ACGGAGA212400.018.15425335
TCGGCGC95200.018.10171345
GCGTTCT221500.017.8838069
Produced by FastQC (version 0.11.5)