FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004853998

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004853998
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47752055
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG823730.17250147663802112No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCACTC396200.016.3466918
AGCTTAG251550.016.22387545
GGTCACT395250.016.2153457
GGGAGGT414650.016.205753
AGGTCAC404350.016.16236
GGCATAG252750.016.10058835
GGAGGTC416550.015.7314184
GAGGTCA424350.015.5463845
ATAGGGG266050.015.51384138
ACTCTCC419400.015.3237211
GAGCTTA275900.014.91891844
TGGCATA278450.014.61503134
TCACTCT457800.014.2648439
CACTCTC455600.014.10172710
GTTGTCG26550.014.06863926
TGGGAGG322700.013.5487452
CATAGGG318100.012.88366537
AGCATAG179000.012.82002245
TAGGGGA509050.012.50015539
TCTCCCC518950.012.40489613
Produced by FastQC (version 0.11.5)