FastQCFastQC Report
Thu 16 Jun 2022
EGAF00004854020

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004854020
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences142999180
Sequences flagged as poor quality0
Sequence length126
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG21141051.4784035824541093Illumina Single End PCR Primer 1 (100% over 50bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8230590.5755690347315278No Hit
GGAAATTAGAAGATAAAAACATACTTTTAGAAGAAAAAAGATAAATTTAA1983220.13868750855774137No Hit
GGGAAATTAGAAGATAAAAACATACTTTTAGAAGAAAAAAGATAAATTTA1728330.12086293082240053No Hit
CCCAAACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTAC1643300.1149167428792249No Hit
CGAAAATGGAAAGATTAATTGGGAGTGGTAGGATGAAACAATTTGGAGAA1568370.109676852692442No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)