FastQCFastQC Report
Mon 4 Jul 2022
EGAF00004854032

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004854032
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences101871407
Sequences flagged as poor quality0
Sequence length126
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2716950.2667038848300191No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG2070130.20321011174411285Illumina Single End PCR Primer 1 (100% over 50bp)
CCCAAACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTAC1610280.158069869399173No Hit
CTAAATACTACCGTATGGCCCACCATAATTACCCCCATACTCCTTACACT1328500.13040950735077214No Hit
CAAACATATAACTGAACTCCTCACACCCAATTGGACCAATCTATCACCCT1026370.10075152883674218No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)