FastQCFastQC Report
Tue 28 Jun 2022
EGAF00004854212

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004854212
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences146578806
Sequences flagged as poor quality0
Sequence length126
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA11178250.7626102507616278No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG5061360.34529957898551855Illumina Single End PCR Primer 1 (100% over 50bp)
CCCAAACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTAC2962660.2021206258154402No Hit
CTAAATACTACCGTATGGCCCACCATAATTACCCCCATACTCCTTACACT2850360.19445921806731048No Hit
CGCAAAACTAACCCCCTAATAAAATTAATTAACCACTCATTCATCGACCT2428800.16569926214298675No Hit
CCGTCATCTACTCTACCATCTTTGCAGGCACACTCATCACAGCGCTAAGC2154610.14699328359926742No Hit
CACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCC1905120.1299724054240147No Hit
GTAAAACCCAGCCCATGACCCCTAACAGGGGCCCTCTCAGCCCTCCTAAT1634860.11153454204013641No Hit
CAAACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTACCC1630850.11126096906533677No Hit
CTAGCATTTACCATCTCACTTCTAGGAATACTAGTATATCGCTCACACCT1570300.10713008536854914No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)