FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004858381

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004858381
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11853883
Sequences flagged as poor quality0
Sequence length65
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGCGC4500.015.0765127
TATGCCG6150.012.47097146
GCGACGT9000.012.45466822
CGAATCG2201.2212599E-412.0687973
CCGACAT15150.011.8840081
CGCTATG9500.011.80129
ACGTTCG4305.675247E-1011.6633856
CCCGTAT11650.011.6540711
GTCGCTA9700.011.5565127
CGACGTC10400.011.06157123
TCGCTAT10550.010.62568728
GACGTCG11000.010.45821324
CTCGTTT25200.010.3068941
CGAGCGA10650.010.24852853
TCGCATT14350.010.0738112
CGACAAT9400.010.0477281
ACGTCGC12050.010.03654325
CGCATCG8450.09.77601738
CCGCTAT4257.73156E-79.7226541
CGCGAGT2759.570904E-49.659521
Produced by FastQC (version 0.11.5)