FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004858383

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004858383
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11592877
Sequences flagged as poor quality0
Sequence length65
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG8450.020.2475946
TCGTATG10700.015.99047243
CGTATGC12650.013.058844
TTCGCGC3655.366019E-1012.9303557
CCCGTAT11600.012.4688631
CGCATCG7600.012.42192838
CGCTATG8150.012.30756329
GATCGGA14300.012.1788061
ACGTTCG3751.1010343E-811.8012396
GTCGGTT15300.011.3828061
CTCGTAT15350.011.33897542
CCGACAT14750.010.8066351
TAACGCC9100.010.69850929
CTCGTTT23650.010.609051
CCGTATC15900.010.5767192
CTACCGA6700.010.566359520
CGATTAG14600.010.3058764
GCGACGT9800.010.23391422
TTACGGC5502.9467628E-1010.19140740
CGTATCG10000.010.0310543
Produced by FastQC (version 0.11.5)