FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004858384

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004858384
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12177542
Sequences flagged as poor quality0
Sequence length65
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG8300.022.0357348
TCGTATG8550.021.0461345
CGTATGC11400.015.78453246
CCCGTAT11700.014.8837341
CGCTAGT2351.0660533E-613.8156271
ACGTTCG3502.746674E-1013.4870686
CTCGTAT13250.013.13554944
CGTATCG12700.012.7768723
GTATCGA10050.012.6231574
CCGTATC16550.011.5874082
GTCGGTT16550.011.23539451
TATCGAA11700.011.095135
GTCGCAT6400.011.0682011
CCCGTCT28050.010.6275771
ACACGTC18550.010.17695413
CGAGCGA11750.010.04232453
ATCGAAG25050.010.0109836
ACGTCTG20400.09.97701615
CCGCAAT9950.09.7889611
CGCTATG9350.09.78144829
Produced by FastQC (version 0.11.5)