FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004858385

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004858385
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13693938
Sequences flagged as poor quality0
Sequence length65
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG9050.018.9057548
TCGTATG11200.016.32989545
CGTATGC11600.014.74958346
TCCGTAT12200.013.78410837
CGCTATG11550.012.77174229
CGTTACG2351.6226833E-512.5542136
CTCGTAT14000.012.22117344
GTCGCTA11950.012.09598127
GTCCGAT12100.011.9526651
CCCGTAT12200.011.8546921
CGCGTAT2503.0457011E-511.8063061
GTCGAAT10400.011.6360231
TATCTCG15750.011.23859941
GTCGCAT7700.011.1163271
GCGACGT12800.011.06166322
CCGACAT20900.011.0154531
GTCGCTT16600.010.8461541
GTTCCGT16800.010.7126535
CGACGTC13800.010.68744123
TCGCTAT13700.010.33600328
Produced by FastQC (version 0.11.5)