FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004858386

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004858386
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11506367
Sequences flagged as poor quality0
Sequence length65
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGTAT10250.013.5333961
CGCATCG5650.013.05455338
TATGCCG7300.012.93126348
CGCTATG8700.012.88632729
TTCGCGC4751.8189894E-1212.4199557
GTCGGTT18650.012.3437911
GTCGCTA9050.012.06075627
TCGTATG7600.012.03251145
CGCTAAT3001.2684814E-611.8057291
ACGTTCG3607.9242454E-811.4728036
TAACGCC7300.011.31618329
TCGCTAT10250.011.22491128
CGACCGC7400.011.16166155
TATGCGA2951.3635181E-510.9990279
CGACCGT8100.010.56313632
CGTATGC9250.010.52398946
CGTATCG9300.010.4682723
CGACGTC10150.010.4621323
CCGACAT17500.010.4565031
GCGACGT10250.010.36014922
Produced by FastQC (version 0.11.5)