FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004858387

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004858387
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12873153
Sequences flagged as poor quality0
Sequence length65
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG9600.015.36413548
TCGTATG11050.014.415745
CGTATGC11500.013.33858546
ACCCGTC11200.013.17104832
CTCGCGT5050.012.8584021
CGCTATG10950.012.663156529
CCCGTAT11450.012.1156871
GCGACGT11000.012.06726622
CGTCCCG12350.011.94468635
CCGACAT16700.011.6649571
CGCATCG7100.011.21920238
GTCGGTT18300.010.9676481
GTCGCTA11900.010.90737127
CTCGTAT15350.010.56964244
CGACGTC12300.010.55204523
CTCGTTT26600.010.5413841
ACGTTCG3952.8519025E-710.4565696
CCGGGAT20650.010.2912491
GACGTCG12400.010.22910224
TCGCTAT13000.010.211596528
Produced by FastQC (version 0.11.5)