FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004858389

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004858389
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14891484
Sequences flagged as poor quality0
Sequence length65
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG12950.017.31247748
TCGTATG14300.015.88442545
CGTATGC16450.013.44968746
CGTGAAA18000.012.94880833
CGTACGA3706.6393113E-1012.75630654
AACGATC18200.012.31965238
AAACGAT19850.012.18739937
GTCGGTT19400.011.4097141
TGAAACG20700.011.40247635
GAAACGA20100.011.30245536
AACGCAT18050.011.27672251
CTCGCGT5800.011.1946341
CTCCGAT25700.011.1391951
CTCGTAT20600.011.02660544
CTCGACT16250.010.8971551
CTCAGAT68950.010.7865241
GTCGCTA11000.010.72703727
TTCGCGC6051.8189894E-1210.7264247
GATCGGA20450.010.390931
CGACGTC11650.010.38115423
Produced by FastQC (version 0.11.5)