FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004858390

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004858390
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24951195
Sequences flagged as poor quality0
Sequence length65
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGTAT25800.017.618081
CGTATCG24050.015.334443
CCGTATC30350.014.5817872
GTATCGA20850.014.0088384
CGTTACG5450.012.4510026
TATCGAA29950.012.3136325
GTCGGTT39100.011.7007171
GTCCGAT22000.011.4039111
CGCGTAA10800.011.205151
CTCGTAT25200.011.0099371
CGAGCGA21800.010.96069153
ATCGAAG56250.010.8048236
CCCGTCT56750.010.766191
CGACCGC16600.010.48444755
CCGGCAT31550.010.3843951
CTCGGAT57250.010.0534861
CTCGAAT43500.010.0422271
CTCGGTT42000.09.979221
CGATTGG16150.09.8649344
ACGTCGC19850.09.80774925
Produced by FastQC (version 0.11.5)