FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004858391

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004858391
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27258023
Sequences flagged as poor quality0
Sequence length65
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGTAT25900.016.6379091
CGTATCG26600.013.30948453
TATGCCG16950.013.05265946
TTCGCGC9150.012.5726797
CCGTATC32900.012.2854722
TCGTATG17750.012.1322143
CGATTGG19350.012.0450264
CCGGTAT21100.011.7501261
GTCCGAT24250.011.6843661
GTATCGA24400.011.6076154
CCCGTCT67050.011.136981
CCGATTG22150.011.0551833
GTCGGTT43800.010.7818071
CTCGTTT50200.010.7595231
CTCGGAT61950.010.5292321
TCCGATT42850.010.2589432
CCGGTTT32600.010.0496531
CTCGTAT28800.09.9408831
CTCGACT32700.09.9286591
CTCGGTT45800.09.9243241
Produced by FastQC (version 0.11.5)