FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004858392

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004858392
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15322525
Sequences flagged as poor quality0
Sequence length65
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGTAT14700.017.8688771
CGCGTAT2302.7685019E-916.6816921
CCGTATC17300.015.1774342
CGTATCG14900.014.4538163
TTCGCGC6550.014.411087
GTATCGA13150.014.3582074
ACGTCGC11300.012.7910325
CGCTAAT4253.45608E-1112.4999471
GTCGCTA11150.011.90559327
TATCGAA16650.011.8715255
CGCTATG11950.011.85022129
GCGACGT12250.011.7991122
CTCGTTT27450.011.6119721
CTCGTAT16050.011.5848421
TCGCTAT12300.011.51230328
CGACGTC12150.011.41062523
ATCGAAG32950.011.3708856
CTCGGTT28750.011.2922231
CCGACAT22750.011.0271211
CTCGGGT39550.010.8950941
Produced by FastQC (version 0.11.5)