FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004858393

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004858393
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12366717
Sequences flagged as poor quality0
Sequence length65
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG7750.014.84454446
ATGCGCG6450.014.17776344
CTCGTTT25150.014.0823641
CATACGA7400.013.55354557
TCGTATG8800.013.40896743
CGATTGG12150.013.35508254
TACGATA8250.012.51473259
CCGGTTT15050.012.158711
GTCGGTT17100.012.0818711
CCCGTAT12400.011.9009211
CCCGATT8950.011.8716691
GCATACG8700.011.86737156
CGTATGC10350.011.68555144
CTCGCGT5105.456968E-1211.574231
CTCAGAT55200.011.5490681
CTCCGAT22200.011.3005141
GCGCGTC8450.011.17113246
TTACGCA5551.8189894E-1211.1611568
TCCGATT21000.011.0988632
CTCGGAT34900.010.993861
Produced by FastQC (version 0.11.5)