FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004858395

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004858395
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21937804
Sequences flagged as poor quality0
Sequence length65
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGTAT23900.012.8431351
TTACGCG1956.0152914E-412.1016178
CTCGTTT43500.011.5344071
GTCGCTA16650.011.1619527
CCGGTAT20700.011.1214091
CTCGGTT41050.010.92863751
CTCGGAT61700.010.8586621
CGTTACG3852.0047992E-710.7278286
ACGTCGC17050.010.72648325
CGCGTAA10400.010.5003551
CCGTATC26300.010.4323132
ACTATCG9950.010.37673553
GTCGGTT30300.010.3252091
AACGCAT22300.010.31823751
CTCCGAT36550.010.0938821
CGTATCG16150.010.0469593
CCGACAT28350.09.8902241
CGACGTC17350.09.860923
CTCAGAT92550.09.85411551
CCGGTTT24900.09.838171
Produced by FastQC (version 0.11.5)