FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004858397

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004858397
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10846071
Sequences flagged as poor quality0
Sequence length65
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG6700.015.41004446
GTCGCGA2153.9020233E-715.1007031
CCCGTAT9800.013.25163651
GTCCGAT8900.012.6019151
TCGTATG8700.012.54564843
ACGCTTA8900.011.93227144
CGCTAAT3001.268103E-611.8060051
CTCGCGT4002.2519089E-911.8060041
CCGGTTT13000.011.8060041
TCGACCG6250.011.80088730
CGCATCG5055.456968E-1211.68393838
CTCGGAT25200.011.5951821
CGACGTC9300.011.41852923
CCCGTCT24600.011.3980741
GTCGCTA9050.011.08268427
CGTATGC9900.011.02496344
CCGTATC11600.010.9365212
GACGTCG10000.010.9142124
TCCGATT15750.010.6773382
CGACCGT6950.010.612308531
Produced by FastQC (version 0.11.5)