FastQCFastQC Report
Fri 27 May 2022
EGAF00004858401

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004858401
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences136612256
Sequences flagged as poor quality0
Sequence length151
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGACGAACTATCTCGTAT19878741.455121274038546TruSeq Adapter, Index 6 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGACGAACTATCGCGTAT7231740.5293624607150913TruSeq Adapter, Index 6 (97% over 36bp)
CCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCA1760730.12888521510105214No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT1688760.12361702013031686No Hit
CGGGGTCTCGCTATGTTGCCCAGGCTGGAGTGCAGTGGCTATTCACAGGC1440160.10541953131935689No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)