FastQCFastQC Report
Thu 16 Jun 2022
EGAF00004858403

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004858403
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences106930385
Sequences flagged as poor quality0
Sequence length151
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCGGTTTAGGGTACGGTCTATACGGAGGGGCTATTTCCTGGGACAACTT2479150.2318471031409828No Hit
CTGTGTTTTTGCTAAACAGTCGCTACCCCCTGGCTTGTGCCCCCCACACA2341170.21894338077993453No Hit
CCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCA2169650.20290303827111442No Hit
CGAGTATTTAGGCTTGGAGGGTGGTCCCCCCATGTTCAGACAGGATTTCA2079500.19447231953761318No Hit
GGGGACCTTAGCTGTAGGTTAGGGCTGTTTCCCTTTTGACGACGGACCTT2076370.19417960573133633No Hit
GTGGTCTTCCACGACTCGATGATAACTTATCTTGAGGGAGGCTTCCCGCT2046150.19135346795955144No Hit
CACGTGTGTAGCCCAGCGTGTAAGGGCCATGAGGACTTGACGTCATCCCC1935190.18097662324885486No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT1836510.17174818925415822No Hit
CTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACG1743360.16303691415681332No Hit
GTCGGTTTAGGGTACGGCCTATACGGAGGGGCTATTTCCTGGGACAACTT1691720.1582076039471849No Hit
CGGGGTCTCGCTATGTTGCCCAGGCTGGAGTGCAGTGGCTATTCACAGGC1648050.15412363847750105No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCGTTACATCTCGTAT1569610.14678802475086944TruSeq Adapter, Index 20 (97% over 37bp)
CTCGGAATTCCACTCGCCTCTCCAAGATTCAAGCCATCTAGTTTCAAAGG1516980.14186613094117262No Hit
CTGGGAACGTATTCACCGCGGCATGCTGATCCGCGATTACTAGCGATTCC1408800.13174926846097113No Hit
GTTTTTGCTAAACAGTCGCTACCCCCTGGCTTGTGCCCCCCACACATGGT1396980.1306438763874272No Hit
CTTAGCTGTAGGTTAGGGCTGTTTCCCTTTTGACGACGGACCTTAGCACC1386930.12970401256855105No Hit
GGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCA1385390.12955999363511128No Hit
GGATAACTTAATGCGTTAGCTGCGCCACCCAAGTTCCATGAACCCGGACA1372610.1283648235251374No Hit
GGGCTGTTTCCCTTTTGACGACGGACCTTAGCACCCGCCGTCTGTCTGCC1343760.12566680649284112No Hit
CCTGGCTTGTGCCCCCCACACATGGTTGCCCATATGTGGGGCCTCCTTCT1268290.11860894356641473No Hit
CCCACTTAGATACGATTTGGGGACCTTAGCTGTAGGTTAGGGCTGTTTCC1259630.11779907086278611No Hit
GTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGA1247230.11663943789223241No Hit
CTCGATGATAACTTATCTTGAGGGAGGCTTCCCGCTTAGATGCTTTCAGC1246630.11658332661946369No Hit
GCCGAGTTCCTTCAGTACACTTCTCTCAAGCGCCTTGGTATACTCTACCA1226660.11471575642414454No Hit
GGGGTCTCGCTATGTTGCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCG1171460.10955351932942167No Hit
CAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAG1151490.10768594913410252No Hit
CTTAGATACGATTTGGGGACCTTAGCTGTAGGTTAGGGCTGTTTCCCTTT1146560.10722490150951948No Hit
CCGGGTTTCGGGTCTAATCCATCAAACTCAGTCGCCCTGTTCAGACTCGC1145000.10707901220032079No Hit
CCCACATGCTTTCCCACTTAGATACGATTTGGGGACCTTAGCTGTAGGTT1076250.10064959552890415No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)