FastQCFastQC Report
Sat 25 May 2024
EGAF00004858405

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004858405
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences136952934
Total Bases20.6 Gbp
Sequences flagged as poor quality0
Sequence length151
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCACGTTATCTCGTAT10829230.7907263965589815TruSeq Adapter, Index 6 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCACGTTATCGCGTAT3748130.2736801535044149TruSeq Adapter, Index 6 (97% over 37bp)
CCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCA1955530.1427884706727057No Hit
CGGGGTCTCGCTATGTTGCCCAGGCTGGAGTGCAGTGGCTATTCACAGGC1661500.12131905111284437No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT1548760.11308702594133545No Hit

[FAIL]Adapter Content

Adapter graph